A circular, extrachromosomal DNA molecule widely used in molecular biology, particularly for RNA interference (RNAi) studies in the nematode Caenorhabditis elegans, serves as a common cloning vector. It is frequently employed to deliver genes of interest into bacteria for replication and subsequent expression. The vector’s design often incorporates features such as antibiotic resistance genes for selection, a multiple cloning site for easy insertion of target sequences, and promoters that allow for controlled gene expression. For example, researchers might insert a gene encoding a specific protein into this type of vector and then introduce it into E. coli to produce large quantities of the protein.
The widespread adoption of this particular vector stems from its well-characterized properties and its effectiveness in specific applications. Its ease of use, coupled with the availability of extensive resources and protocols, makes it a popular choice for researchers. Its development has significantly facilitated research into gene function and regulation, particularly in model organisms where efficient gene knockdown is crucial. Historically, its introduction into the scientific community accelerated the pace of discovery in related fields.
