Failure to execute the reagent prime function on a Cobas analyzer can lead to inaccurate or unreliable test results. This function is designed to ensure the proper hydration and delivery of reagents to the reaction cuvette. Without adequate priming, inconsistencies in reagent concentration and volume can occur, directly affecting the accuracy of the assay. For example, if the reagent lines are not fully purged of air bubbles or stagnant fluid, the initial reaction may be compromised, leading to skewed measurements.
The priming process is crucial for maintaining the integrity of the diagnostic testing process. It prevents carryover contamination between different tests, ensuring that results reflect the true analyte concentration in the sample. Historically, inadequate reagent preparation has been a significant source of error in clinical laboratories. Modern automated analyzers incorporate priming cycles to mitigate these risks, improving the overall quality and reliability of diagnostic testing. A properly executed prime reduces the likelihood of erroneous flags, reruns, and ultimately, incorrect patient diagnoses.
